Simultaneous action of microbial phospholipase C and lipase on model bacterial membranes – Modeling the processes crucial for bioaugmentation

Bioaugmentation is a promising method of the remediation soils polluted by persistent organic pollutants (POP). Unfortunately, it happens frequently that microorganisms inoculated into soil die out due to presence enzymes secreted autochthonous microorganisms. Especially destructive are here phospholipases C (PLC) and lipases which destruct microorganism's cellular membrane. The composition bacterial membranes differs between species, so highly possible depending on membrane constitution some bacteria more resistant PLCs than other. To shed light these problems we applied phospholipid Langmuir monolayers as model microbial studied their interactions with ?-toxin (model PLC) lipase isolated from fungus Candida rugosa. Membrane phospholipids differing in headgroup (phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols cardiolipins) tail structure were applied. characterized technique, visualized Brewster angle microscopy, packing mode molecules was verified application diffraction synchrotron radiation. We also mutual miscibility diacylglycerols native interaction crucial for understanding PLC activity. It turned all investigated classes can be hydrolyzed PLC; however, they differ profoundly hydrolysis degree. Depending effects initial action organization diacylglycerol ruin or completely neutral them.